Simultaneous visualization of the subfemtomolar expression of microRNA and microRNA target gene using HILO microscopy† †Electronic supplementary information (ESI) available: The LED device for the sample photobleaching, a schematic presentation of HILO microscopy, fluorescence spectra and hybridization curves of the molecular beacons, the linear correlation between the miRNA fluorescence intensity and the miRNA copy number, a validation of the miRNA adsorption and miRNA target gene expression via RT-qPCR, a validation of RT-qPCR using capillary electrophoresis, the reproducibility of RT-qPCR and Poisson distribution of the miRNA pipetting as well as a complete list of the oligonucleotides used in this study. See DOI: 10.1039/c7sc02701j Click here for additional data file.
نویسندگان
چکیده
Department of Chemistry, Tunghai Univ [email protected] Graduate Institute of Oncology, College o Taipei 100, Taiwan Department of Internal Medicine, National Taiwan Department of Oncology, National Taiwan † Electronic supplementary information sample photobleaching, a schematic uorescence spectra and hybridization linear correlation between the miRNA copy number, a validation of the miRN expression via RT-qPCR, a validat electrophoresis, the reproducibility of RT miRNA pipetting as well as a complete li study. See DOI: 10.1039/c7sc02701j Cite this: Chem. Sci., 2017, 8, 6670
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